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anti human flt 3 ligand flt3l antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti human flt 3 ligand flt3l antibody
    Anti Human Flt 3 Ligand Flt3l Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab308/Human+Flt-3+Ligand%2FFLT3L+Antibody/pm38701783-1106-22-27
    Average 92 stars, based on 3 article reviews
    anti human flt 3 ligand flt3l antibody - by Bioz Stars, 2026-09
    92/100 stars

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    Article Title: Single-cell RNA-seq reveals AML hierarchies relevant to disease progression and immunity
    Article Snippet: Cell cycle analysis was performed as follows: (1) stain MUTZ-3 cells with CD14-PE-Cy7 (Coulter A22331) and CD34-FITC (BD Pharmingen 348053) antibodies; (2) spin and permeabilize using Phosphoflow Perm 2 solution (BD 347692 diluted 10× in H 2 O); (3) wash with PBS 2% FBS; (4) stain with Ki67 Alexa Fluor 647 (BD Pharmingen 561126); (4) wash; (5) resuspend in Cytofix buffer (BD 554655 diluted 4× in PBS) with DAPI at 1 μg / ml; (5) analysis on an BD LSRII analyzer within 30 minutes. .. Data was analyzed using FlowJo software (Tree Star, Inc.). table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse CD15-V450, clone MMA BD Biosciences Cat# 642917; RRID: AB_1645751 Monoclonal mouse CD34-FITC, clone 8G12 BD Biosciences Cat# 348053; RRID: AB_2228982 Monoclonal mouse CD38-PE, clone HB7 BD Biosciences Cat# 347687; RRID: AB_400341 Mouse CD14-APC, clone RMO52 Beckman Coulter Cat# IM2580U Monoclonal mouse CD11b-APC-Cy7, clone ICRF44 BD Biosciences Cat# 557754; RRID: AB_396860 Monoclonal mouse FLT-3, clone 40406 R & D Systems Cat# MAB308; RRID: AB_2104978 Mouse CD34-APC, clone 8G12 BD Biosciences Cat# 340441; RRID: AB_400514 Anti-Human CD14-PC7 antibody Beckman Coulter Cat# A22331; RRID: AB_10639528 Monoclonal rat CD4-FITC, clone A161A1 BioLegend Cat# 357405; RRID: AB_2562356 Mouse CD33-PE-Cy7 , clone P67.6 BD Biosciences Cat# 333946; RRID: AB_399961 Monoclonal mouse CD69-APC, clone FN50 BioLegend Cat# 310909; RRID: AB_314844 Monoclonal mouse Ki67-Alexa Fluor® 647, clone B56 BD Biosciences Cat# 561126; RRID: AB_10611874 Biological Samples See Table S1 for a list of patients included in the study. .. Recombinant DNA VSV.G Addgene Cat# 14888 dRT-pMDLg/pRRE Addgene Cat# 60488 pRSV-Rev Addgene Cat# 12253 pMAL University of Toronto, Dick lab N/A Software and Algorithms R version 3.4 R Core Team https://www.r-project.org R package - data.table CRAN https://cran.r-project.org/web/packages/data.table/index.html R package - Rsamtools Bioconductor http://bioconductor.org/packages/release/bioc/html/Rsamtools.html R package - Rtsne Github https://github.com/jkrijthe/Rtsne R package - randomForest CRAN https://cran.r-project.org/web/packages/randomForest/index.html STAR version 2.5.2b Github https://github.com/alexdobin/STAR SPRING Kleintools (Weinreb et al., 2018) https://kleintools.hms.harvard.edu/tools/spring.html BackSPIN Github (Zeisel et al., 2015) https://github.com/linnarsson-lab/BackSPIN BWA-MEM Cornell University https://arxiv.org/abs/1303.3997 FlowJo version 10.4.2 TreeStar https://www.flowjo.com Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/prism/ Integrative Genomics Viewer (IGV version 2.4.8) Broad Institute http://software.broadinstitute.org/software/igv/ download Albacore version 2.3.3 Github https://github.com/dvera/albacore SC3 Bioconductor http://bioconductor.org/packages/release/bioc/html/SC3.html Open in a separate window KEY RESOURCES TABLE Immunohistochemistry Slides were run on the Bond III Imunostainer (deparaffinized on the machine) by Leica (Buffalo Grove, IL).



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